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Temporomandibular Joint Arthroplasty with Human Amniotic Membrane - Case Report

Bauer F, Hingsammer LM, Wolff KD, Kesting MR. 30 November 2014

 2013;13:e17. Epub 2013 Mar 18.

Abstract

This case reports the usage of human amniotic membrane combined with a costochondral graft as an interpositional material in temporomandibular joint reconstruction for the first time in humans. Because of the favorable outcome 20 months postoperatively, it has to be considered as an approach bringing to light the antiadhesive potential of amniotic membrane. This case report must be regarded as initial spadework and should motivate other institutions to intensify their clinical research in this field. Because of the fact that currently used interpositional materials do not prevent the recurrence of temporomandibular joint ankylosis sufficiently, it is of great interest to establish a proper therapeutic intervention fulfilling these demands. Furthermore, the demonstrated antiadhesive properties of amniotic membrane highlight its multifaceted field of application. Nevertheless, further studies have to prove the findings reported in our case.

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Temporomandibular Joint Arthroplasty with Human Amniotic Membrane - Case Report

Bauer F, Hingsammer LM, Wolff KD, Kesting MR. 18 March 2013

 2013;13:e17. Epub 2013 Mar 18.

Abstract

This case reports the usage of human amniotic membrane combined with a costochondral graft as an interpositional material in temporomandibular joint reconstruction for the first time in humans. Because of the favorable outcome 20 months postoperatively, it has to be considered as an approach bringing to light the antiadhesive potential of amniotic membrane. This case report must be regarded as initial spadework and should motivate other institutions to intensify their clinical research in this field. Because of the fact that currently used interpositional materials do not prevent the recurrence of temporomandibular joint ankylosis sufficiently, it is of great interest to establish a proper therapeutic intervention fulfilling these demands. Furthermore, the demonstrated antiadhesive properties of amniotic membrane highlight its multifaceted field of application. Nevertheless, further studies have to prove the findings reported in our case.

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Journal of Neurosurgery

Giovanna Squintani, Bruno Bonetti, Adolfo Paolin, Daniela Vici, Elisa Cogliati, Bruno Murer, Giorgio Stevanato 26 July 2013

Nerve regeneration across cryopreserved allografts from cadaveric donos: a novel approach for peripheral nerve reconstruction

Abstract

Object. The use of allografts from cadaveric donors has attracted renewed interest in recent years, and pretreatment with cryopreservation adn immunosuppression methods has been investigated to maximize axonal regrowth and minimize allograft rejection. The authors wanted to assess the outcome of treatments of brachial plexus stretch injuries with cryopreserved allografts from cadaveric donors in nonimmunosuppressed patients.

Methods. Ten patients with brachial plexus lesions were submitted to electromyography (EMG) testing 1 and 3 months after a traumatic event and 1 week before surgery to localize and identify the type of lesion. Intraoperative EMG recordings were performed for intraoperative monitoring to select the bet surgical strategy, and postoperative EMG was used to follow up patients and determine surgical outcomes. If nerve action potentials (NAPs) were present intraoperatively, neurolysis was performed, whereas muscular/nerve neurotization was performed if NAPs were absent. Cryopreserved allografts obtained from selected cadaveric donors and provided by the tissue bank of Treviso were used for nerve reconstruction in patients who were not treated with immunosuppressive drugs.

Results. The surgical strategy was selected according to the type and site of the nerve lesion and on the basis of IOM results: 14 cryopreserved allografts were used for 7 muscular neurotizations and for 7 nerve neurotizations, and 5 neurolysis procedures were performed. All of the patients had regained motor function at the 1- and 2-year follow-ups.

Conclusions. Some variables may affect functional recovery after allograft surgery, and the outcome of peripheral nerve reconstruction is more favorable when patients are carefully evaluated and selected for the surgery. The authors demonstrated that using cryopreserved allografts from cadaveric donors is a valid surgical strategy to restore function of the damaged nerve without the need for any immunosuppressive treatments. This approach offers new perspectives on procedures for extensive reconstruction of brachial and lumbosacral plexures.

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European Heart Journal

E. Sommariva, S. Brambilla, C. Carbuccicchio, E. Gambini, V. Meraviglia, A. Dello Russo, F.M. Farina, M. Casella, V. Catto, G. Pontone, M. Chiesa, I. Stadiotti, E. Cogliati, A. Paolin, N. Ouali Alami, C. Preziuso, G. d'Amati, G.I. Colombo, A. Rossini, M.C. Capogrossi, C. Tondo, G. Pompilio 20 November 2015

Cardiac mesenchymal stromal cells are a source of adipocytes in arrhythmogenic cardiomyopathy

Abstract

Arrhythmogenic cardiomyopathy (ACM) is a genetic disorder mainly due to mutations in desmosomal genes, characterized by progressive fibro-adipose replacement of the myocardium, arrhythmias, and sudden death. It is still unclear which cell type is responsible for fibro-adipose substitution and which molecular mechanisms lead to this structural change. Cardiac mesenchymal stromal cells (C-MSC) are the most abundant cells in teh heart, with propensity to differentiate into several cell types, including adipocytes, and their role in ACM is unknown. The aim of the present study was to investigate whther C-MSC contributed to excess adipocytes in patients with ACM.

We found that, in ACM patients' explanted heart sections, cells actively differentiating into adipocytes are of mesenchymal origin. Therefore, we isolated C-MSC from endomyocardial biopsies of ACM and from not affected by arrhythmogenic cardiomyopathy (NON-ACM) (control) patients. We found that both ACM adn control C-MSC express desmosomal genes, with ACM C-MSC showing lower expression of plakophilin (PKP2) protein vs controls. Arrhythmogenic cardiomyopathy C-MSC cultured in adipogenic medium accumulated more lipid droplets than controls. Accordingly, the expression of adipogenic genes was higher in ACM vs. NON-ACM C-MSC, while expression of cell cycle and anti-adipogenic genes was lower. Both lipid accumulation and transcription reprogramming were dependent on PKP2 deficiency.

Cardiac mesenchymal stromal cells contribute to the adipogenic substitution observed in ACM patients' hearts. Moreover, C-MSC from ACM patients recapitulate the features of ACM adipogenensis, representing a novel, scalable, patient-specific in vitro tool for future mechanistic studies.

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Cell and Tissue Banking

Adolfo Paolin . Diletta Trojan . Antonio Leonardi . Stefano Mellone . Antonio Volpe . Augusto Orlandi . Elisa Cogliati 12 April 2016

Cytokine expression and ultrastructural alterations in freshfrozen, freeze-dried and c-irradiated human amniotic membranes

Abstract

The aim of this work was to compare the effects on human amniotic membrane of freeze-drying and c-irradiation at doses of 10, 20 and 30 kGy, with freezing. For this purpose, nine cytokines (interleukin 10, platelet-derived growth factor-AA, plateletderived growth factor-BB, basic fibroblast growth factor, epidermal growth factor, transforming growth factor beta 1, and tissue inhibitors of metalloproteinase-1, -2, and -4) were titrated in 5 different preparations for each of 3 amniotic membranes included in the study. In addition, the extracellular matrix structure of each sample was assessed by transmission electron microscopy. While freeze-drying did not seem to affect the biological structure or cytokine content of the different amniotic membrane samples, c-irradiation led to a significant decrease in the tissue inhibitors of metalloproteinase-4, basic fibroblast growth factor and epidermal growth factor, and induced structural damage to the epithelium, basement membrane and lamina densa. The higher the irradiation dose the more severe the damage to the amniotic membrane structure. In conclusion, the Authors recommend processing amniotic membrane under sterile conditions to guarantee safety at every step rather than final sterilization with c-irradiation, thereby avoiding alteration to the biological characteristics of the amniotic membrane.

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Cell and Tissue Banking

Adolfo Paolin, Elisa Cogliati, Diletta Trojan, Carlo Griffoni, Andrea Grassetto, Hossein Mostafa Elbadawy, Diego Ponzin 11 July 2015

Amniotic membranes in ophthalmology: long term data on transplantation outcomes

Abstract

The use of amniotic membrane (AM) is a widespread clinical practice for eye surgeries and the treatment of an increasing number of ocular surface pathologies. Here we describe the AM collection methods and donor selection criteria adopted by our tissue bank to distribute 5349 amniotic membrane patches over the last 12 years for the treatment of several ocular pathologies. Specific quality control measures are described and the long term results attained using the reported procedure are presented. A case of AM utilized to treat severe ocular ulceration is also described as an example of AM transplantation. Collective data for the total amniotic membrane patches deployed to treat various ocular diseases are discussed and success rates for AM transplantations are reported. An extensive follow-up is illustrated. The results suggest that the procedures and protocols used by the Treviso Tissue Bank Foundation and Veneto Eye Bank Foundation for collection, rpeservation, distribution and follow-up are of an optimal standard. Accordingly, the authors conclude that the safety and efficiency of the proposed procedure for the therapeutic use of AM to trat various ocular pathologies are reproducible, with additional evidence favoring the use of AM as an alternative to conventional medical treatment for certain ocular conditions.

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Cell and Tissue Banking

Adolfo Paolin . Diletta Trojan . Antonio Leonardi . Stefano Mellone . Antonio Volpe . Augusto Orlandi . Elisa Cogliati 02 February 2017

Cytokine expression and ultrastructural alterations in freshfrozen, freeze-dried and c-irradiated human amniotic membranes

Abstract

The aim of this work was to compare the effects on human amniotic membrane of freeze-drying and c-irradiation at doses of 10, 20 and 30 kGy, with freezing. For this purpose, nine cytokines (interleukin 10, platelet-derived growth factor-AA, plateletderived growth factor-BB, basic fibroblast growth factor, epidermal growth factor, transforming growth factor beta 1, and tissue inhibitors of metalloproteinase-1, -2, and -4) were titrated in 5 different preparations for each of 3 amniotic membranes included in the study. In addition, the extracellular matrix structure of each sample was assessed by transmission electron microscopy. While freeze-drying did not seem to affect the biological structure or cytokine content of the different amniotic membrane samples, c-irradiation led to a significant decrease in the tissue inhibitors of metalloproteinase-4, basic fibroblast growth factor and epidermal growth factor, and induced structural damage to the epithelium, basement membrane and lamina densa. The higher the irradiation dose the more severe the damage to the amniotic membrane structure. In conclusion, the Authors recommend processing amniotic membrane under sterile conditions to guarantee safety at every step rather than final sterilization with c-irradiation, thereby avoiding alteration to the biological characteristics of the amniotic membrane.

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Cell and Tissue Banking

Adolfo Paolin, Elisa Cogliati, Diletta Trojan, Carlo Griffoni, Andrea Grassetto, Hossein Mostafa Elbadawy, Diego Ponzin 11 July 2015

Amniotic membranes in ophthalmology: long term data on transplantation outcomes

Abstract

The use of amniotic membrane (AM) is a widespread clinical practice for eye surgeries and the treatment of an increasing number of ocular surface pathologies. Here we describe the AM collection methods and donor selection criteria adopted by our tissue bank to distribute 5349 amniotic membrane patches over the last 12 years for the treatment of several ocular pathologies. Specific quality control measures are described and the long term results attained using the reported procedure are presented. A case of AM utilized to treat severe ocular ulceration is also described as an example of AM transplantation. Collective data for the total amniotic membrane patches deployed to treat various ocular diseases are discussed and success rates for AM transplantations are reported. An extensive follow-up is illustrated. The results suggest that the procedures and protocols used by the Treviso Tissue Bank Foundation and Veneto Eye Bank Foundation for collection, rpeservation, distribution and follow-up are of an optimal standard. Accordingly, the authors conclude that the safety and efficiency of the proposed procedure for the therapeutic use of AM to trat various ocular pathologies are reproducible, with additional evidence favoring the use of AM as an alternative to conventional medical treatment for certain ocular conditions.

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Cell and Tissue Banking

Alessandro Viscioni, Maurizio Franco, Adolfo Paolin, Elisa Cogliati, Maura Callegari, Ilaria Zollino, Vincenzo Sollazzo, Francesco Carinci 06 July 2010

Effectiveness of fresh frozen and cryopreserved homologue iliac crest grafts used in sinus lifting: a comparative study

Abstract

In the last decade, several investigators have reported that autologous and homologous fresh frozen bones (FFB) are effective materials to restore alveolar ridges previous to insert dental implants. Recently we have used cryopreserved homologue grafts (CFFB). Here we reported a retrospective comparative study between implants inserted in FFB and CFFB evaluate their clinical outcome. Patients were treated with a split mouth scheme for bone grafting with FFB and CFFB and spiral family implants (SPI) were inserted int eh same surgical time. Several variables (patient, grafts, anatomic site, implant, prosthetic restoration) were investigated. Implant' failure and peri-implant bone resorption were considered as predictor of clinical outcome. A total fo 84 SFIs were inserted in 12 patients. Implants were inserted to replace 8 incisors, 4 cuspids, 31 premolars and 41 molars. The mean follow-up was 14 months. Three out of 84 implants was lost (i.e. survival rate SVR = 96.4%) and no differences were detected among the studied variables. Similar result was obtained by analyzing the crestal bone resorption around implant' neck (i.e. success rate). FFB and CFFB have high and comparable survival and success rate. Implants inserted with one step surgical procedure in native (i.e. not grafted) bone, FFB and CFFB have similar clinical outcome.

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Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 21 May 2015

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

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Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 28 February 2015

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

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Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 31 August 2014

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

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Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 30 June 2015

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 05 November 2011

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 30 September 2010

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 30 September 2010

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 31 August 2013

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 05 April 2004

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 30 November 2010

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

Download abstract

Amnion membrane for coverage of gingival recession: A novel application

Rucha Shah, N. K. Sowmya, and D. S. Mehta 17 April 2014

Abstract

Amnion allograft has been used in the field of medicine for its exceptional wound-modulating properties. However, in the field of dentistry, only a limited number of reports have explored its potential in healing of oral wounds.

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