Questo sito utilizza cookie tecnici e consente l'invio di cookie di "terze parti" al fine di effettuare misure di traffico per la valutazione delle prestazioni del sito. Per saperne di più, clicca sull'apposito bottone "Maggiori dettagli", che aprirà in modalità pop up l'informativa estesa, dove è possibile avere informazioni per negare il consenso all'installazione di qualunque cookie. Cliccando il bottone OK, o cliccando su una qualsiasi altra area del sito, presterai consenso all'uso dei cookie.

A new method to repair recto-vaginal fistula: Use of human amniotic membrane in an animal model

A new method to repair recto-vaginal fistula: Use of human amniotic membrane in an animal model

Reza Roshanravan, Leila Ghahramani, Massood Hosseinzadeh, Mastoureh Mohammadipour, Sam Moslemi, Abbas Rezaianzadeh, Ali Reza Safarpour, Salar Rahimikazerooni, and Seyed Vahid Hosseini 17 April 2014

Abstract

Recto-vaginal fistula is primarily one of the co-morbidities of vaginal delivery. These patients suffer from persistent malodor vaginal discharge. Various surgical techniques have been employed by surgeons in the course of time. This is the first trial of applying Human Amniotic Membrane (HAM) as a bio-prosthesis in repairing recto-vaginal fistula. In a prospective animal study, 8 mixed-breed female dogs weighing 23-27 kg with the age of 12-18 months were selected. They were randomly divided into two groups for standard recto-vaginal fistula repair and fistula repair with human amniotic membrane. The Kruskal-Wallis and Mann Whitney tests were performed to indicate statistical differences. After 6 weeks, fistulas were evaluated both grossly and microscopically. In gross examination, there were no difference between the two groups and healing of fistula seemed to have been occurred in all dogs expect for one which had a persistent patent fistulous tract. Microscopic healing was scored according to epithelialization, collagenization inflammation, ulcer and necrosis of samples. Healing score was significantly higher in the HAM group than the standard group. Our findings revealed that using HAM as a bio-prosthesis to repair recto-vaginal fistula would result in better surgical and histological outcomes comparing to simple repair.

 

Download abstract

Comparison of a poly-L-lactide-co-1- caprolactone and human amniotic membrane for urothelium tissue engineering applications

Comparison of a poly-L-lactide-co-1- caprolactone and human amniotic membrane for urothelium tissue engineering applications

Reetta Sartoneva, Suvi Haimi, Susanna Miettinen, Bettina Mannerstro, Anne-Marie Haaparanta, George K. Sa´ndor, Minna Kelloma, Riitta Suuronen and Tuija Lahdes-Vasama 30 November 2010

Abstract

The reconstructive surgery of urothelial defects, such as severe hypospadias is susceptible to complications. The major problem is the lack of suitable grafting materials. Therefore, finding alternative treatments such as reconstruction of urethra using tissue engineering is essential. The aim of this study was to compare the effects of naturally derived acellular human amniotic membrane (hAM) to synthetic poly-L-lactide-co-1-caprolactone (PLCL) on human urothelial cell (hUC) viability, proliferation and urothelial differentiation level. The viability of cells was evaluated using live/dead staining and the proliferation was studied using WST-1 measurement. Cytokeratin (CK)7/8 and CK19 were used to confirm that the hUCs maintained their phenotype on different biomaterials. On the PLCL, the cell number significantly increased during the culturing period, in contrast to the hAM, where hUC proliferation was the weakest at 7 and 14 days. In addition, the majority of cells were viable and maintained their phenotype when cultured on PLCL and cell culture plastic, whereas on the hAM, the viability of hUCs decreased with time and the cells did not maintain their phenotype. The PLCL membranes supported the hUC proliferation significantly more than the hAM. These results revealed the significant potential of PLCL membranes in urothelial tissue engineering applications.

Download abstract

Dehydrated Human Amnion/Chorion Membrane Allograft Nerve Wrap Around the Prostatic Neurovascular Bundle Accelerates Early Return to Continence and Potency Following Robot-assisted Radical Prostatectomy: Propensity Score–matched Analysis

Dehydrated Human Amnion/Chorion Membrane Allograft Nerve Wrap Around the Prostatic Neurovascular Bundle Accelerates Early Return to Continence and Potency Following Robot-assisted Radical Prostatectomy: Propensity Score–matched Analysis

Vipul R. Patel, Srinivas Samavedi, Anthony S. Bates, Anup Kumar, Rafael Coelho, Bernardo Rocco, Kenneth Palmer 20 May 2013

European Association of Urology (2015) Article in press

We present a propensity-matched analysis of patients undergoing placement of dehydrated human amnion/chorion membrane (dHACM) around the neurovascular bundle (NVB) during nerve-sparing (NS) robot-assisted laparoscopic prostatectomy (RARP).

Download abstract

Early experience in reconstruction of long ureteral strictures with allogenic amniotic membrane

Early experience in reconstruction of long ureteral strictures with allogenic amniotic membrane

Koziak A1, Salagierski M, Marcheluk A, Szczesniewski R, Sosnowski M. 17 April 2014

Abstract

To present our experience with the application of human amniotic membrane for the reconstruction of extensive ureteral wall defects. Between 2003 and 2006, 11 patients underwent reconstructive surgery of the ureter. A human amniotic membrane allograft was used to supplement ureteral wall defects. Indications for the procedure included ureteral strictures of a 5.5 cm average (range, 3-8 cm) localized in different parts of the ureter: upper (5), middle (5) and lower (3). The etiology of ureteral loss was: postinflammatory after a complicated stone disease (5), iatrogenic (4) and idiopathic (2). Diagnosis of ureteral stricture was based on antegrade pyelography and excretory urography. Two patients had synchronous treatment for upper and middle ureteral stenosis. Treatment efficacy was assessed by excretory urography and ultrasound. The mean hospitalization time was 11.9 days, mean operation time 128 min and with an average follow up of 25.2 months. Complications included: stricture recurrence (1) and symptomatic urinary tract infections (2). Excretory urography showed lack of obstruction and normal width of ureters. In one patient, residual hydronephrosis was present on ultrasound. The described method seems to be a promising tool in the reconstruction of extensive ureteral strictures.

Download abstract

Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndrome

Functional and anatomic results of amnion vaginoplasty in young women with Mayer-Rokitansky-Küster-Hauser syndrome

Fotopoulou C, Sehouli J, Gehrmann N, Schoenborn I, Lichtenegger W. 20 May 2015

Fertil Steril (2010)

To evaluate the surgical outcome and the long-term anatomic and functional results in young women with Mayer-Rokitansky-Küster-Hauser Syndrome (MRKH) undergoing neovaginal creation with amniotic membranes. Evaluation of surgical and functional outcome according to clinical records and validated questionnaires about sexuality (Female Sexual Function Index [FSFI]) over a 1.5-year follow-up period.

Download abstract

Secondary Vaginal Atresia Treated with Vaginoplasty Using Amnion Graft: A Case Report

Secondary Vaginal Atresia Treated with Vaginoplasty Using Amnion Graft: A Case Report

Setu Rathod, Sunil Kumar Samal 20 May 2015

Journal of Clinical and Diagnostic Research. 2014 Nov, Vol-8(11): OD05-OD06

We report a case of 26-year-old primipara with last child birth 1.5 year back who presented with amenorrhoea, cyclical lower abdominal pain and dyspareunia for past six months. Examination revealed vaginal atresia secondary to previous traumatic/mismanaged vaginal delivery with haematocolpos. She was managed by vaginoplasty with amnion graft and regular dilation with soft vaginal mould for six weeks and was advised for regular intercourse after that.

Download abstract